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A comparison of two agar gel immunodiffusion methods and a complement fixation test for serologic diagnosis of Brucella ovis infection in experimentally infected rams Arq. Bras. Med. Vet. Zootec.
Xavier,M.N.; Sant'Anna,F.M.; Silva,T.M.A.; Costa,E.A.; Moustacas,V.S.; Merlo,F.A.; Carvalho Júnior,C.A.; Dasso,M.G.; Mathias,L.A.; Gouveia,A.M.G.; Lage,A.P.; Santos,R.L..
A infecção por Brucella ovis é considerada uma das principais causas de epididimite e infertilidade em carneiros, resultando em falhas reprodutivas e perdas econômicas significativas em rebanhos ovinos ao redor do mundo. O estudo teve o objetivo de avaliar três testes sorológicos disponíveis para o diagnóstico da brucelose ovina por B. ovis, utilizando 181 soros ovinos. Amostras de soro provenientes de carneiros experimentalmente infectados foram coletadas ao longo de 192 dias pós-infecção (n=117) e durante o período pré-infecção (n=9). Adicionalmente, amostras de soro foram obtidas de ovinos provenientes de um rebanho livre para B. ovis (n=55). As técnicas de imunodifusão em gel de agar (IDGA), utilizando dois antígenos disponíveis comercialmente, e de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Brucella ovis; Serology; Complement fixation; Immunodiffusion.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400031
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Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep R. Bras. Zootec.
Ruiz,Ana Zuley.
The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cloning; NMDA receptor; Ovis aries; Ram; Sequencing.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000400183
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Comparison of sexual performance and testicular characteristics of melatonin treated Kivircik and Charollais rams during the non-breeding season Arq. Bras. Med. Vet. Zootec.
Cevik,M.; Yilmazer,C.; Kocyigit,A..
ABSTRACT This study was conducted to investigate the effects of melatonin implantation on basic testicular characteristics and reproductive performance of Kivircik and Charollais rams and ewes during the non-breeding season. In this study, 8 Kivircik and 6 Charollais rams were used. Rams were implanted with 54 mg melatonin according to the manufacturer's instructions. At melatonin implantation and at ram introduction the reproductive performance and testicular characteristics were evaluated. Throughout the experimental period, rams were permanently kept outdoors under conditions of natural day length and at ambient temperature. The effects of exogenous melatonin treatments on the reproductive performances of rams and ewes, estrus response, pregnancy rate,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Melatonin; Fertility; Non-breeding season.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200278
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Effect of diets containing whole cottonseed on the quality of sheep semen - doi: 10.4025/actascianimsci.v34i3.12963 Animal Sciences
Gomes, Maria das Graças; Empresa Estadual de Pesquisa Agropecuária da Paraíba S. A.; Mazzoni Gonzalez, Carmen Iara; Empresa Estadual de Pesquisa Agropecuária da Paraíba S. A.; Carvalho, Francisco Fernando Ramos de; Universidade Federal Rural de Pernambuco; Soares, Adriana Trindade; Empresa Estadual de Pesquisa Agropecuária da Paraíba S. A..
This study evaluated the semen quality of Santa Ines sheep fed diets containing different levels of whole cottonseed (WCS). Twenty sheep were distributed into four groups that were given diets with 0; 20; 30; 40% of WCS. For this study the semen was collected by electroejaculation, and a monthly andrological examination was performed during nine months. The analyzed variables were: scrotal circumference (SC), appearance and color of the ejaculate, progressive motility (PM), vigor, mass movement (MM), total sperm concentration (spzX109), sperm morphology (SM) and blood hematocrit. The SC had no difference among diets, with mean 31.83 cm. The animals fed diets with WCS, presented mean intake of 9.16 mg gossypol kg BW, and were prone to present greenish...
Tipo: Pesquisa de campo; análise laboratorial Palavras-chave: 50400002- 50403001 andrological; Gossypol; Ram; Reproduction; Sperm motility.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12963
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Guia prático para seleção visual de matrizes e carneiros. Infoteca-e
RETORE, M.; CORREA, E. B..
Padrão Racial; Idade; Oclusões Defeituosas; Cascos; Escore de Condição Corporal; Peso; Aprumos; Coloração da Mucosa Ocular; Aspecto Físico; Úbere; Testículos; Prepúcio; Pênis; Libido; Estrutura Óssea; Ausência de Doenças Infecciosa.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Ram; Ovinoculture; Brasil.; Matriz; Ovinocultura..
Ano: 2013 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/966388
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Intake and digestibility of sorghum (Sorghum bicolor, L. Moench) silages with different tannin contents in sheep R. Bras. Zootec.
Teixeira,Alex de Matos; Ribeiro Junior,Gabriel de Oliveira; Velasco,Frederico Osório; Faria Júnior,Wilson Gonçalves de; Rodriguez,Norberto Mario; Santos Rodrigues,José Avelino; McAllister,Tim; Gonçalves,Lúcio Carlos.
The purpose of this study was to evaluate the voluntary intake and digestibility of three sorghum (Sorghum bicolor, L. Moench) hybrid silages in sheep. The hybrids used were H1 -BRS 655 (CMSXS 222 A × CMSXS 235 R), with tannin; H2 -(ATF54 A × CMSXS 235 R), without tannin; and H3 -BRS 610 (CMSXS 232 A × CMSXS 234 R), without tannin. The intake and digestibility of dry matter (DM), gross energy (GE), neutral detergent fiber (NDF), acid detergent fiber (ADF) and crude protein (CP) were measured. Eighteen crossbred sheep weighing 59.4 kg (±8.3) were used in the trial. A completely randomized design with three treatments (hybrids) and six repetitions (sheep) was used. There were no differences in the DM intake or apparent digestibility among the hybrids. Silage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forage preservation; Nitrogen balance; Nutritional value; Ram; Sorghum hybrid.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000100003
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Sperm Motility and Viability in West African Dwarf Rams Treated with Euphorbia hirta International Journal of Morphology
Oyeyemi,M. O; Olukole,S. G; Taiwo,Bolanle; Adeniji,Deborah A.
Four adult sexually matured and clinically healthy West African Dwarf (WAD) rams aged between 24 and 30 months were used for the study. The rams were first used as control and later as experimental animals upon being orally dosed with Euphorbia hirta extract at 400mg/kg body weight for 14 days. Semen samples were collected from the rams a day after the administration of the plant extra and seven days after. The objective of the study was to investigate the effect of Euphorbia hirta on the semen picture of WAD rams. There were significantly differences (P <0.05) in the semen picture as reflected in a reduction of sperm motility from 80% to 47.5% and live-dead ratio from 90.75% to 32.5% in the control and post-experimental stages of the study...
Tipo: Journal article Palavras-chave: Sperm motility; Ram; Viability; Euphorbia hirta.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200024
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Viability of ovine spermatozoa collected from epididymides stored at 18°-25°C for 48 hours post mortem Arq. Bras. Med. Vet. Zootec.
Bergstein-Galan,T.G.; Weiss,R.R.; Barbosa,T.S.R.; Kozicki,L.E.; Bicudo,S.D..
ABSTRACT The objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25°C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0±1.9%) compared to AV (86.4±1.0%). PM decreased (P<0.05) from 12 hours after death (31.3±4.0%) compared to AV group (73.2±1.4%). The percentage of viable cells in HOST decreased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Epididymis; Room temperature.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401023
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